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How to Write Hypothesis for Lab Report
- How to Write Hypothesis for…
What Is a Real Hypothesis?
A hypothesis is a tentative statement that proposes a possible explanation for some phenomenon or event. A useful hypothesis is a testable statement that may include a prediction.
When Are Hypotheses Used?
The keyword is testable. That is, you will perform a test of how two variables might be related. This is when you are doing a real experiment. You are testing variables. Usually, a hypothesis is based on some previous observations such as noticing that in November many trees undergo color changes in their leaves and the average daily temperatures are dropping. Are these two events connected? How?
Any laboratory procedure you follow without a hypothesis is really not an experiment. It is just an exercise or demonstration of what is already known.
How Are Hypotheses Written?
- Chocolate may cause pimples.
- Salt in soil may affect plant growth.
- Plant growth may be affected by the color of the light.
- Bacterial growth may be affected by temperature.
- Ultraviolet light may cause skin cancer.
- The temperature may cause leaves to change color.
All of these are examples of hypotheses because they use the tentative word “may.”. However, their form is not particularly useful. Using the word may do not suggest how you would go about proving it. If these statements had not been written carefully, they may not have even been hypotheses at all. For example, if we say “Trees will change color when it gets cold.” we are making a prediction. Or if we write, “Ultraviolet light causes skin cancer.” could be a conclusion. One way to prevent making such easy mistakes is to formalize the form of the hypothesis.
Formalized Hypotheses example: If the incidence of skin cancer is related to exposure levels of ultraviolet light , then people with a high exposure to uv light will have a higher frequency of skin cancer.
If leaf color change is related to temperature , then exposing plants to low temperatures will result in changes in leaf color .
Notice that these statements contain the words, if and then. They are necessary for a formalized hypothesis. But not all if-then statements are hypotheses. For example, “If I play the lottery, then I will get rich.” This is a simple prediction. In a formalized hypothesis, a tentative relationship is stated. For example, if the frequency of winning is related to the frequency of buying lottery tickets . “Then” is followed by a prediction of what will happen if you increase or decrease the frequency of buying lottery tickets. If you always ask yourself that if one thing is related to another, then you should be able to test it.
Formalized hypotheses contain two variables. One is “independent” and the other is “dependent.” The independent variable is the one you, the “scientist” control, and the dependent variable is the one that you observe and/or measure the results. In the statements above the dependent variable is underlined and the independent variable is underlined and italicized .
The ultimate value of a formalized hypothesis is it forces us to think about what results we should look for in an experiment.
For the “ If, Then, Because ” hypothesis…you would use: “ IF pigs and humans share the same nutritional behaviors, THEN their internal organs should look relatively the same BECAUSE of similar function and composure.” That is an example. For the “If, Then, Because” you should follow this guideline:
IF X and Y both do or share this, THEN this should be found/confirmed, BECAUSE of this fact or logical assumption.
Example Question : How does the type of liquid (water, milk, or orange juice) given to a plant affect how tall the plant will grow? Hypothesis : If the plant is given water then the plant will grow the tallest because water helps the plant absorb the nutrients that the plant needs to survive.
Author: William Anderson (Schoolworkhelper Editorial Team)
Tutor and Freelance Writer. Science Teacher and Lover of Essays. Article last reviewed: 2020 | St. Rosemary Institution © 2010-2022 | Creative Commons 4.0
How would I write a hypothesis about a flying pig lab?
@keisha your lab hypothesis should have been written before the experiment. The purpose of the hypothesis was to create a testable statement in which your experimental data would either support or reject. Having a hypothesis based on a logical assumption (regardless of whether your data supports it) is still correct. If there is a disagreement between your hypothesis and experimental data it should be addressed in the discussion. So you can go ahead an choose a hypothesis for either increase or decrease of adipogenesis after the inducement of insulin and not be wrong….as long as it is correctly formatted (see … Read more »
Hey, I am having trouble writing my hypothesis.. I am supposed to write a hypothesis about how much adipogenesis was produced after the inducement of insulin. However, after proceeding with the experiments the results were On/Off .. meaning it will increase, decrease, increase, etc.. so it wasnt a constant result. It was supposed to be increasing.
please help!!!
this is very helpful but i don’t know how i would structure my hypothesis. i’m supposed to come up with a hypothesis related to the topic ‘how does mass effect the stopping distance of a cart?’. Could you help?
Thank you so much, it really help alot.:)
This is a rather difficult usage of this construct. It would most likely follow
“If the empirical formula of (enter compound’s name) is (enter compound’s formula) then it would be expected that combustion of _________ would yield _________, because (enter your rationale)
Need more background info.
For the “If, then, because” hypothesis I am doing an experiment to determine the empirical formula by using combustion but I am unsure on how to formulate the hypothesis using this structure.
For the “If, Then, Because” hypothesis…you would use: “IF pigs and humans share the same nutritional behaviors, THEN their internal organs should look relatively the same BECAUSE of similar function and composure.” That is an example. For the “If, Then, Because” you should follow this guideline:
Thanks, really helpful. Just one question, what about the ‘because’ part? right after the ‘if’ and ‘then’ parts?
I really need help for onion skin lab hypothesis for class
@Lauren An if/and statement is not usually apart of the convention. What exactly do you need help with?
Is there such thing as a if/and statement? I am in 8th grade science an I need to know for my lab report due tomorrow.HELP!!!!
Would have been better if more examples were given
If the purpose of your lab is “To obtain dissecting skills in an observational lab,” you can’t really formulate a testable hypothesis for that. I’ll assume you are doing some kind of pig or frog dissection. Often teachers give general outlines of skills that students are meant to ascertain from an experiment which aren’t necessarily what the actual experiment is directly testing. Obviously to do the dissection lab you need to obtain dissection skills but testing that would be rather subjective unless the teacher provided you with standards or operationally defined “dissecting skills”. If I were you, I would obviously … Read more »
Thanks for this, it proved to be helpful. However, I do have a few questions. Obviously different teachers or instructors have their own requirements for their classes. How would you write an appropriate Question to follow each purpose in your lab report? For example: If the purpose was, “To obtain dissecting skills in an observational lab,” what question could you formulate with the purpose? (which is answered in the hypothesis) And if a teacher requires the hypothesis to be in the format “If, Then, Because” how should this be written? I can actively complete the if and then, but I’m … Read more »

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How to Write a Lab Report
Lab reports describe your experiment.
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Lab reports are an essential part of all laboratory courses and usually a significant part of your grade. If your instructor gives you an outline for how to write a lab report, use that. Some instructors require a lab report to be included in a lab notebook , while others will request a separate report. Here's a format for a lab report you can use if you aren't sure what to write or need an explanation of what to include in the different parts of the report.
A lab report is how you explain what you did in your experiment, what you learned, and what the results meant.
Lab Report Essentials
Not all lab reports have title pages, but if your instructor wants one, it would be a single page that states:
- The title of the experiment.
- Your name and the names of any lab partners.
- Your instructor's name.
- The date the lab was performed or the date the report was submitted.
The title says what you did. It should be brief (aim for ten words or less) and describe the main point of the experiment or investigation. An example of a title would be: "Effects of Ultraviolet Light on Borax Crystal Growth Rate". If you can, begin your title using a keyword rather than an article like "The" or "A".
Introduction or Purpose
Usually, the introduction is one paragraph that explains the objectives or purpose of the lab. In one sentence, state the hypothesis. Sometimes an introduction may contain background information, briefly summarize how the experiment was performed, state the findings of the experiment, and list the conclusions of the investigation. Even if you don't write a whole introduction, you need to state the purpose of the experiment, or why you did it. This would be where you state your hypothesis .
List everything needed to complete your experiment.
Describe the steps you completed during your investigation. This is your procedure. Be sufficiently detailed that anyone could read this section and duplicate your experiment. Write it as if you were giving direction for someone else to do the lab. It may be helpful to provide a figure to diagram your experimental setup.
Numerical data obtained from your procedure usually presented as a table. Data encompasses what you recorded when you conducted the experiment. It's just the facts, not any interpretation of what they mean.
Describe in words what the data means. Sometimes the Results section is combined with the Discussion.
Discussion or Analysis
The Data section contains numbers; the Analysis section contains any calculations you made based on those numbers. This is where you interpret the data and determine whether or not a hypothesis was accepted. This is also where you would discuss any mistakes you might have made while conducting the investigation. You may wish to describe ways the study might have been improved.
Conclusions
Most of the time the conclusion is a single paragraph that sums up what happened in the experiment, whether your hypothesis was accepted or rejected, and what this means.
Figures and Graphs
Graphs and figures must both be labeled with a descriptive title. Label the axes on a graph, being sure to include units of measurement. The independent variable is on the X-axis, the dependent variable (the one you are measuring) is on the Y-axis. Be sure to refer to figures and graphs in the text of your report: the first figure is Figure 1, the second figure is Figure 2, etc.
If your research was based on someone else's work or if you cited facts that require documentation, then you should list these references.
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How to Develop a Good Research Hypothesis

The story of a research study begins by asking a question. Researchers all around the globe are asking curious questions and formulating research hypothesis. However, whether the research study provides an effective conclusion depends on how well one develops a good research hypothesis. Research hypothesis examples could help researchers get an idea as to how to write a good research hypothesis.
This blog will help you understand what is a research hypothesis, its characteristics and, how to formulate a research hypothesis
Table of Contents
What is Hypothesis?
Hypothesis is an assumption or an idea proposed for the sake of argument so that it can be tested. It is a precise, testable statement of what the researchers predict will be outcome of the study. Hypothesis usually involves proposing a relationship between two variables: the independent variable (what the researchers change) and the dependent variable (what the research measures).
What is a Research Hypothesis?
Research hypothesis is a statement that introduces a research question and proposes an expected result. It is an integral part of the scientific method that forms the basis of scientific experiments. Therefore, you need to be careful and thorough when building your research hypothesis. A minor flaw in the construction of your hypothesis could have an adverse effect on your experiment. In research, there is a convention that the hypothesis is written in two forms, the null hypothesis, and the alternative hypothesis (called the experimental hypothesis when the method of investigation is an experiment).

Essential Characteristics of a Good Research Hypothesis
As the hypothesis is specific, there is a testable prediction about what you expect to happen in a study. You may consider drawing hypothesis from previously published research based on the theory.
A good research hypothesis involves more effort than just a guess. In particular, your hypothesis may begin with a question that could be further explored through background research.
To help you formulate a promising research hypothesis, you should ask yourself the following questions:
- Is the language clear and focused?
- What is the relationship between your hypothesis and your research topic?
- Is your hypothesis testable? If yes, then how?
- What are the possible explanations that you might want to explore?
- Does your hypothesis include both an independent and dependent variable?
- Can you manipulate your variables without hampering the ethical standards?
- Does your research predict the relationship and outcome?
- Is your research simple and concise (avoids wordiness)?
- Is it clear with no ambiguity or assumptions about the readers’ knowledge
- Is your research observable and testable results?
- Is it relevant and specific to the research question or problem?

The questions listed above can be used as a checklist to make sure your hypothesis is based on a solid foundation. Furthermore, it can help you identify weaknesses in your hypothesis and revise it if necessary.
Source: Educational Hub
How to formulate an effective research hypothesis.
A testable hypothesis is not a simple statement. It is rather an intricate statement that needs to offer a clear introduction to a scientific experiment, its intentions, and the possible outcomes. However, there are some important things to consider when building a compelling hypothesis.
1. State the problem that you are trying to solve.
Make sure that the hypothesis clearly defines the topic and the focus of the experiment.
2. Try to write the hypothesis as an if-then statement.
Follow this template: If a specific action is taken, then a certain outcome is expected.
3. Define the variables
Independent variables are the ones that are manipulated, controlled, or changed. Independent variables are isolated from other factors of the study.
Dependent variables , as the name suggests are dependent on other factors of the study. They are influenced by the change in independent variable.
4. Scrutinize the hypothesis
The types of research hypothesis are stated below:
1. Simple Hypothesis
It predicts the relationship between a single dependent variable and a single independent variable.
2. Complex Hypothesis
It predicts the relationship between two or more independent and dependent variables.

3. Directional Hypothesis
It specifies the expected direction to be followed to determine the relationship between variables and is derived from theory. Furthermore, it implies the researcher’s intellectual commitment to a particular outcome.
4. Non-directional Hypothesis
It does not predict the exact direction or nature of the relationship between the two variables. The non-directional hypothesis is used when there is no theory involved or when findings contradict previous research.
5. Associative and Causal Hypothesis
The associative hypothesis defines interdependency between variables. A change in one variable results in the change of the other variable. On the other hand, the causal hypothesis proposes an effect on the dependent due to manipulation of the independent variable.
6. Null Hypothesis
Null hypothesis states a negative statement to support the researcher’s findings that there is no relationship between two variables. There will be no changes in the dependent variable due the manipulation of the independent variable. Furthermore, it states results are due to chance and are not significant in terms of supporting the idea being investigated.
7. Alternative Hypothesis
It states that there is a relationship between the two variables of the study and that the results are significant to the research topic. An experimental hypothesis predicts what changes will take place in the dependent variable when the independent variable is manipulated. Also, it states that the results are not due to chance and that they are significant in terms of supporting the theory being investigated.
Research Hypothesis Examples of Independent and Dependent Variables:
Research Hypothesis Example 1 The greater number of coal plants in a region (independent variable) increases water pollution (dependent variable). If you change the independent variable (building more coal factories), it will change the dependent variable (amount of water pollution).
Research Hypothesis Example 2 What is the effect of diet or regular soda (independent variable) on blood sugar levels (dependent variable)? If you change the independent variable (the type of soda you consume), it will change the dependent variable (blood sugar levels)
You should not ignore the importance of the above steps. The validity of your experiment and its results rely on a robust testable hypothesis. Developing a strong testable hypothesis has few advantages, it compels us to think intensely and specifically about the outcomes of a study. Consequently, it enables us to understand the implication of the question and the different variables involved in the study. Furthermore, it helps us to make precise predictions based on prior research. Hence, forming a hypothesis would be of great value to the research. Here are some good examples of testable hypotheses.
More importantly, you need to build a robust testable research hypothesis for your scientific experiments. A testable hypothesis is a hypothesis that can be proved or disproved as a result of experimentation.
Importance of a Testable Hypothesis
To devise and perform an experiment using scientific method, you need to make sure that your hypothesis is testable. To be considered testable, some essential criteria must be met:
- There must be a possibility to prove that the hypothesis is true.
- There must be a possibility to prove that the hypothesis is false.
- The results of the hypothesis must be reproducible.
Without these criteria, the hypothesis and the results will be vague. As a result, the experiment will not prove or disprove anything significant.
What are your experiences with building hypotheses for scientific experiments? What challenges did you face? How did you overcome these challenges? Please share your thoughts with us in the comments section.

Wow! You really simplified your explanation that even dummies would find it easy to comprehend. Thank you so much.
Thanks a lot for your valuable guidance.
I enjoy reading the post. Hypotheses are actually an intrinsic part in a study. It bridges the research question and the methodology of the study.
Useful piece!
This is awesome.Wow.
It very interesting to read the topic, can you guide me any specific example of hypothesis process establish throw the Demand and supply of the specific product in market
Nicely explained
It is really a useful for me Kindly give some examples of hypothesis
It was a well explained content ,can you please give me an example with the null and alternative hypothesis illustrated
clear and concise. thanks.
So Good so Amazing
Good to learn
Thanks a lot for explaining to my level of understanding
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How To Write A Lab Report | Step-by-Step Guide & Examples
Published on May 20, 2021 by Pritha Bhandari . Revised on July 15, 2022.
A lab report conveys the aim, methods, results, and conclusions of a scientific experiment. The main purpose of a lab report is to demonstrate your understanding of the scientific method by performing and evaluating a hands-on lab experiment. This type of assignment is usually shorter than a research paper .
Lab reports are commonly used in science, technology, engineering, and mathematics (STEM) fields. This article focuses on how to structure and write a lab report.
Table of contents
Structuring a lab report, introduction, frequently asked questions about lab reports.
The sections of a lab report can vary between scientific fields and course requirements, but they usually contain the purpose, methods, and findings of a lab experiment .
Each section of a lab report has its own purpose.
- Title: expresses the topic of your study
- Abstract : summarizes your research aims, methods, results, and conclusions
- Introduction: establishes the context needed to understand the topic
- Method: describes the materials and procedures used in the experiment
- Results: reports all descriptive and inferential statistical analyses
- Discussion: interprets and evaluates results and identifies limitations
- Conclusion: sums up the main findings of your experiment
- References: list of all sources cited using a specific style (e.g. APA )
- Appendices : contains lengthy materials, procedures, tables or figures
Although most lab reports contain these sections, some sections can be omitted or combined with others. For example, some lab reports contain a brief section on research aims instead of an introduction, and a separate conclusion is not always required.
If you’re not sure, it’s best to check your lab report requirements with your instructor.
Your title provides the first impression of your lab report – effective titles communicate the topic and/or the findings of your study in specific terms.
Create a title that directly conveys the main focus or purpose of your study. It doesn’t need to be creative or thought-provoking, but it should be informative.
- The effects of varying nitrogen levels on tomato plant height.
- Testing the universality of the McGurk effect.
- Comparing the viscosity of common liquids found in kitchens.
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An abstract condenses a lab report into a brief overview of about 150–300 words. It should provide readers with a compact version of the research aims, the methods and materials used, the main results, and the final conclusion.
Think of it as a way of giving readers a preview of your full lab report. Write the abstract last, in the past tense, after you’ve drafted all the other sections of your report, so you’ll be able to succinctly summarize each section.
To write a lab report abstract, use these guiding questions:
- What is the wider context of your study?
- What research question were you trying to answer?
- How did you perform the experiment?
- What did your results show?
- How did you interpret your results?
- What is the importance of your findings?
Nitrogen is a necessary nutrient for high quality plants. Tomatoes, one of the most consumed fruits worldwide, rely on nitrogen for healthy leaves and stems to grow fruit. This experiment tested whether nitrogen levels affected tomato plant height in a controlled setting. It was expected that higher levels of nitrogen fertilizer would yield taller tomato plants.
Levels of nitrogen fertilizer were varied between three groups of tomato plants. The control group did not receive any nitrogen fertilizer, while one experimental group received low levels of nitrogen fertilizer, and a second experimental group received high levels of nitrogen fertilizer. All plants were grown from seeds, and heights were measured 50 days into the experiment.
The effects of nitrogen levels on plant height were tested between groups using an ANOVA. The plants with the highest level of nitrogen fertilizer were the tallest, while the plants with low levels of nitrogen exceeded the control group plants in height. In line with expectations and previous findings, the effects of nitrogen levels on plant height were statistically significant. This study strengthens the importance of nitrogen for tomato plants.
Your lab report introduction should set the scene for your experiment. One way to write your introduction is with a funnel (an inverted triangle) structure:
- Start with the broad, general research topic
- Narrow your topic down your specific study focus
- End with a clear research question
Begin by providing background information on your research topic and explaining why it’s important in a broad real-world or theoretical context. Describe relevant previous research on your topic and note how your study may confirm it or expand it, or fill a gap in the research field.
This lab experiment builds on previous research from Haque, Paul, and Sarker (2011), who demonstrated that tomato plant yield increased at higher levels of nitrogen. However, the present research focuses on plant height as a growth indicator and uses a lab-controlled setting instead.
Next, go into detail on the theoretical basis for your study and describe any directly relevant laws or equations that you’ll be using. State your main research aims and expectations by outlining your hypotheses .
Based on the importance of nitrogen for tomato plants, the primary hypothesis was that the plants with the high levels of nitrogen would grow the tallest. The secondary hypothesis was that plants with low levels of nitrogen would grow taller than plants with no nitrogen.
Your introduction doesn’t need to be long, but you may need to organize it into a few paragraphs or with subheadings such as “Research Context” or “Research Aims.”
A lab report Method section details the steps you took to gather and analyze data. Give enough detail so that others can follow or evaluate your procedures. Write this section in the past tense. If you need to include any long lists of procedural steps or materials, place them in the Appendices section but refer to them in the text here.
You should describe your experimental design, your subjects, materials, and specific procedures used for data collection and analysis.
Experimental design
Briefly note whether your experiment is a within-subjects or between-subjects design, and describe how your sample units were assigned to conditions if relevant.
A between-subjects design with three groups of tomato plants was used. The control group did not receive any nitrogen fertilizer. The first experimental group received a low level of nitrogen fertilizer, while the second experimental group received a high level of nitrogen fertilizer.
Describe human subjects in terms of demographic characteristics, and animal or plant subjects in terms of genetic background. Note the total number of subjects as well as the number of subjects per condition or per group. You should also state how you recruited subjects for your study.
List the equipment or materials you used to gather data and state the model names for any specialized equipment.
List of materials
35 Tomato seeds
15 plant pots (15 cm tall)
Light lamps (50,000 lux)
Nitrogen fertilizer
Measuring tape
Describe your experimental settings and conditions in detail. You can provide labelled diagrams or images of the exact set-up necessary for experimental equipment. State how extraneous variables were controlled through restriction or by fixing them at a certain level (e.g., keeping the lab at room temperature).
Light levels were fixed throughout the experiment, and the plants were exposed to 12 hours of light a day. Temperature was restricted to between 23 and 25℃. The pH and carbon levels of the soil were also held constant throughout the experiment as these variables could influence plant height. The plants were grown in rooms free of insects or other pests, and they were spaced out adequately.
Your experimental procedure should describe the exact steps you took to gather data in chronological order. You’ll need to provide enough information so that someone else can replicate your procedure, but you should also be concise. Place detailed information in the appendices where appropriate.
In a lab experiment, you’ll often closely follow a lab manual to gather data. Some instructors will allow you to simply reference the manual and state whether you changed any steps based on practical considerations. Other instructors may want you to rewrite the lab manual procedures as complete sentences in coherent paragraphs, while noting any changes to the steps that you applied in practice.
If you’re performing extensive data analysis, be sure to state your planned analysis methods as well. This includes the types of tests you’ll perform and any programs or software you’ll use for calculations (if relevant).
First, tomato seeds were sown in wooden flats containing soil about 2 cm below the surface. Each seed was kept 3-5 cm apart. The flats were covered to keep the soil moist until germination. The seedlings were removed and transplanted to pots 8 days later, with a maximum of 2 plants to a pot. Each pot was watered once a day to keep the soil moist.
The nitrogen fertilizer treatment was applied to the plant pots 12 days after transplantation. The control group received no treatment, while the first experimental group received a low concentration, and the second experimental group received a high concentration. There were 5 pots in each group, and each plant pot was labelled to indicate the group the plants belonged to.
50 days after the start of the experiment, plant height was measured for all plants. A measuring tape was used to record the length of the plant from ground level to the top of the tallest leaf.
In your results section, you should report the results of any statistical analysis procedures that you undertook. You should clearly state how the results of statistical tests support or refute your initial hypotheses.
The main results to report include:
- any descriptive statistics
- statistical test results
- the significance of the test results
- estimates of standard error or confidence intervals
The mean heights of the plants in the control group, low nitrogen group, and high nitrogen groups were 20.3, 25.1, and 29.6 cm respectively. A one-way ANOVA was applied to calculate the effect of nitrogen fertilizer level on plant height. The results demonstrated statistically significant ( p = .03) height differences between groups.
Next, post-hoc tests were performed to assess the primary and secondary hypotheses. In support of the primary hypothesis, the high nitrogen group plants were significantly taller than the low nitrogen group and the control group plants. Similarly, the results supported the secondary hypothesis: the low nitrogen plants were taller than the control group plants.
These results can be reported in the text or in tables and figures. Use text for highlighting a few key results, but present large sets of numbers in tables, or show relationships between variables with graphs.
You should also include sample calculations in the Results section for complex experiments. For each sample calculation, provide a brief description of what it does and use clear symbols. Present your raw data in the Appendices section and refer to it to highlight any outliers or trends.
The Discussion section will help demonstrate your understanding of the experimental process and your critical thinking skills.
In this section, you can:
- Interpret your results
- Compare your findings with your expectations
- Identify any sources of experimental error
- Explain any unexpected results
- Suggest possible improvements for further studies
Interpreting your results involves clarifying how your results help you answer your main research question. Report whether your results support your hypotheses.
- Did you measure what you sought out to measure?
- Were your analysis procedures appropriate for this type of data?
Compare your findings with other research and explain any key differences in findings.
- Are your results in line with those from previous studies or your classmates’ results? Why or why not?
An effective Discussion section will also highlight the strengths and limitations of a study.
- Did you have high internal validity or reliability?
- How did you establish these aspects of your study?
When describing limitations, use specific examples. For example, if random error contributed substantially to the measurements in your study, state the particular sources of error (e.g., imprecise apparatus) and explain ways to improve them.
The results support the hypothesis that nitrogen levels affect plant height, with increasing levels producing taller plants. These statistically significant results are taken together with previous research to support the importance of nitrogen as a nutrient for tomato plant growth.
However, unlike previous studies, this study focused on plant height as an indicator of plant growth in the present experiment. Importantly, plant height may not always reflect plant health or fruit yield, so measuring other indicators would have strengthened the study findings.
Another limitation of the study is the plant height measurement technique, as the measuring tape was not suitable for plants with extreme curvature. Future studies may focus on measuring plant height in different ways.
The main strengths of this study were the controls for extraneous variables, such as pH and carbon levels of the soil. All other factors that could affect plant height were tightly controlled to isolate the effects of nitrogen levels, resulting in high internal validity for this study.
Your conclusion should be the final section of your lab report. Here, you’ll summarize the findings of your experiment, with a brief overview of the strengths and limitations, and implications of your study for further research.
Some lab reports may omit a Conclusion section because it overlaps with the Discussion section, but you should check with your instructor before doing so.
A lab report conveys the aim, methods, results, and conclusions of a scientific experiment . Lab reports are commonly assigned in science, technology, engineering, and mathematics (STEM) fields.
The purpose of a lab report is to demonstrate your understanding of the scientific method with a hands-on lab experiment. Course instructors will often provide you with an experimental design and procedure. Your task is to write up how you actually performed the experiment and evaluate the outcome.
In contrast, a research paper requires you to independently develop an original argument. It involves more in-depth research and interpretation of sources and data.
A lab report is usually shorter than a research paper.
The sections of a lab report can vary between scientific fields and course requirements, but it usually contains the following:
- Abstract: summarizes your research aims, methods, results, and conclusions
- References: list of all sources cited using a specific style (e.g. APA)
- Appendices: contains lengthy materials, procedures, tables or figures
The results chapter or section simply and objectively reports what you found, without speculating on why you found these results. The discussion interprets the meaning of the results, puts them in context, and explains why they matter.
In qualitative research , results and discussion are sometimes combined. But in quantitative research , it’s considered important to separate the objective results from your interpretation of them.
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Formatting a testable hypothesis
What Is a Real Hypothesis?
A hypothesis is a tentative statement that proposes a possible explanation to some phenomenon or event. A useful hypothesis is a testable statement, which may include a prediction. A hypothesis should not be confused with a theory. Theories are general explanations based on a large amount of data. For example, the theory of evolution applies to all living things and is based on wide range of observations. However, there are many things about evolution that are not fully understood such as gaps in the fossil record. Many hypotheses have been proposed and tested.
When Are Hypotheses Used?
The key word is testable . That is, you will perform a test of how two variables might be related. This is when you are doing a real experiment. You are testing variables. Usually, a hypothesis is based on some previous observation such as noticing that in November many trees undergo color changes in their leaves and the average daily temperatures are dropping. Are these two events connected? How?
Any laboratory procedure you follow without a hypothesis is really not an experiment. It is just an exercise or demonstration of what is already known.
How Are Hypotheses Written?
- Chocolate may cause pimples.
- Salt in soil may affect plant growth.
- Plant growth may be affected by the color of the light.
- Bacterial growth may be affected by temperature.
- Ultra violet light may cause skin cancer.
- Temperature may cause leaves to change color.
All of these are examples of hypotheses because they use the tentative word "may”. However, their form is not particularly useful. Using the word “may” does not suggest how you would go about providing supporting evidence for the hypothesis. If these statements had not been written carefully, they may not have even been hypotheses at all. For example, if we say "Trees will change color when it gets cold." we are making a prediction. Or if we write, "Ultraviolet light causes skin cancer." could be a conclusion. One way to prevent making such easy mistakes is to formalize the form of the hypothesis.
Formalized Hypotheses example: If skin cancer is related to ultraviolet light , then people with a high exposure to uv light will have a higher frequency of skin cancer .
If leaf color change is related to temperature , then exposing plants to low temperatures will result in changes in leaf color.
Notice that these statements contain the words , if and then . They are necessary in a formalized hypothesis. But not all if-then statements are hypotheses. For example, "If I play the lottery, then I will get rich." This is a simple prediction. In a formalized hypothesis, a tentative relationship is stated. For example, if the frequency of winning is related to frequency of buying lottery tickets. "Then" is followed by a prediction of what will happen if you increase or decrease the frequency of buying lottery tickets. If you always ask yourself that if one thing is related to another, then you should be able to test it.
Formalized hypotheses contain two variables. One is "independent" and the other is "dependent." The independent variable is the one you, the "scientist" control and the dependent variable is the one that you observe and/or measure the results
The ultimate value of a formalized hypothesis is it forces us to think about what results we should look for in an experiment.
Notice there are two parts to a formalized hypothesis: the “if” portion contains the testable proposed relationship and the “then” portion is the prediction of expected results from an experiment. An acceptable hypothesis contains both aspects, not just the prediction portion.
Investigation 1: Conducting a Biological Experiment
Purpose : to learn how to use the scientific method by conducting an experiment.
You have learned so far that scientists use the "scientific method" in solving problems. Although there is no set order to the sequence, a scientific investigation may include some or all of the following activities; literature search, stating the problem, writing a hypothesis, designing an experiment, collecting data/observation, verification, graphing data, interpreting data, and forming a conclusion.
For today’s experiment you will develop a formalized hypothesis based upon the part II experiment of the lab exercise on page 5 of the lab manual (including both testable relationship and prediction). Each lab group will Set-up both experiments; pages 4 and 5 of the lab manual. During the next week each lab group will collect data in tables . Once the data is collected your group will have to summarize the data and represent the results using a table and figure.
Step 1: Introduction material before beginning an experiment.
Refer to page 4 of your lab manual for a description of the experiment. Notice that page 4 includes introductory information that explains the underlying hypothesis of spontaneous generation.
A. State the Problem (this is usually a general question but often does not include a proposed relationship)
What is the general question addressed by Procedure 1?
(notice this is not a formalized hypothesis; it does not contain any information to identify the independent or dependent variable. This step is not included in a formal lab report introduction section.)
B. Literature Search (this section is required to explain the reason for a proposed relationship [the hypothesis])
What background information is required to explain your formalized hypothesis? For the formal lab report you need to find appropriate cited sources to support your hypothesis. ___________________________________________________________________ ___________________________________________________________________
, c. writing a hypothesis, procedure 1 page 4 of the lab manual.
Read the procedure on page 4 of the lab manual to determine the independent and dependent variables . Write a formalized hypothesis. Include the dependent and independent variables.
Procedure 2 page 5 of the lab manual
Read procedure II on page 5 of the lab manual to determine the independent and dependent variables . Write a formalized hypothesis. Include the dependent and independent variables.
1. Are the independent and dependent variables the same for both procedures?
2. Are the hypotheses for procedure 1 and procedure II the same?
If not what relationship does procedure II test?
Experimental Design: Follow the instructions on page 4 and 5 of your lab manual.
The following tables are meant to aid in collecting the raw experimental data. Do not use these tables in the final lab report; follow the example table in the handout on how to write a results section.
*Note this is not the final table to use in your formal lab report.
Data/Observations
Table 1. Time – series data for your own group. Record the dependent variable each assigned day.
Procedure 1: Dependent variable?_________________________
Table 2. Time – series data for your own group. Record the dependent variable each assigned day. Each group must have a sediment score for each treatment for four days; this data will be used to generate a time-series graph for the lab report.
Procedure 2 Dependent variable=__ Sediment Score _
Interpretations and Conclusions
- Why should the results from each group be combined with other class group data? before analyzing the results for a lab report? ___________________________________________________________________
- What was the independent variable of Procedure I?____________ Procedure II? ______________________________ What was the dependent variable of Procedure I?______________ Procedure II?_____________________________
- Why were five Petri dishes necessary Procedure I ? What is the purpose of including the fifth Petri dish if the lid was never removed? ___________________________________________________________________ ___________________________________________________________________

Check the class standard flasks for the sediment scale:
During next week’s lab each group will present their results and fill in a class data table, see below. The class data table is the data that must be used for the Chi-square test in your lab report.
Table 3. Bio 100 class data for Procedure II, page 5 of the lab manual. The sediment scores are based upon the score assigned to each flask on day 5. n= 6.

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Formalized hypotheses contain two variables. One is “independent” and the other is “dependent.” The independent variable is the one you, the “scientist” control
____References or examples to support all statements of fact or opinion. The hypothesis is what you propose will happen in the experiment.
Writing Lab Reports · Why was the research done or experiment conducted? · What problem is being addressed? · What results were found? · What are
The second part of your Introduction is a statement of your hypothesis(es). Hypotheses are educated guesses that you propose as a tentative answer to the
In one sentence, state the hypothesis. Sometimes an introduction may contain background information, briefly summarize how the experiment was
1. State the problem that you are trying to solve. · 2. Try to write the hypothesis as an if-then statement. · 3. Define the variables · 4. Scrutinize the
Description of how to write a hypothesis for a lab report.
o Be prepared for the lab. Before coming to the lab session, read the procedure in the lab manual thoroughly. Write down a purpose, hypothesis, and a general
Your conclusion should be the final section of your lab report. Here, you'll summarize the findings of your experiment, with a brief overview of
But not all if-then statements are hypotheses. For example, "If I play the lottery, then I will get rich." This is a simple prediction. In a formalized